Web Reference: Jul 21, 2025 ยท The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST (Basic Local Alignment Search Tool) is a tool that compares nucleotide or protein sequences to sequence databases. It is extensively used to identify sequence similarity and homology, thereby helping researchers infer the function and evolutionary relationships of sequences and identify the members of gene families. BLAST is also often used as part of other algorithms that require approximate sequence matching. BLAST is available on the web on the NCBI website. Different types of BLASTs are available according to the query sequences and the target databases.
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